Moussa F, Pressac M, Genin E, Roux S, Trivin F, Rassat A, Céolin R, Szwarc H
Jeune Equipe 1990, Faculte de Pharmacie, Tours, France.
J Chromatogr B Biomed Sci Appl. 1997 Aug 15;696(1):153-9. doi: 10.1016/s0378-4347(97)00228-4.
A high-performance liquid chromatographic (HPLC) assay method for C60 fullerene, in blood, liver and spleen using photodiode-array detection or mass spectrometric detection (LC-MS) and C70 fullerene, as the internal standard, is described. The recovery from mouse blood and tissues spiked with micronized C60 exceeds 90%. The method is linear from 0.05 to 200 mg of C60 per liter of blood and from 0.05 to 5.00% of C60 per tissue weight. The limit of detection of the method is 0.1 ng of C60 per injection. This method was applied to mouse blood and tissue samples after intraperitoneal administration of a micronized C60 suspension.
描述了一种用于血液、肝脏和脾脏中C60富勒烯的高效液相色谱（HPLC）测定方法，该方法使用光电二极管阵列检测或质谱检测（LC-MS），并以C70富勒烯作为内标。从小鼠血液和添加了微粉化C60的组织中的回收率超过90%。该方法在每升血液中C60含量为0.05至200毫克以及每个组织重量中C60含量为0.05至5.00%的范围内呈线性。该方法的检测限为每次进样0.1纳克C60。该方法应用于腹腔注射微粉化C60悬浮液后的小鼠血液和组织样本。
