Santa T, Yoshioka D, Homma H, Imai K, Satoh M, Takayanagi I
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Biol Pharm Bull. 1995 Sep;18(9):1171-4. doi: 10.1248/bpb.18.1171.
Fullerence (C60) was determined by high-performance liquid chromatography using both ultraviolet and mass spectrometric detection. The detection limit for each method was 0.05 and 2.0 ng (signal-to-noise ratio (S/N = 2)) per injection, respectively. Rat plasma spiked with C60 (10 micrograms/ml) was extracted using solid phase extraction with a recovery of 62.1% and the coefficient of variation (c.v., n = 5) between intra-day assays was 4.0%. The calibration curve for peak area and plasma C60 concentration with ultraviolet detection showed good linearity (r = 0.996) over the range 0.5-60 micrograms/ml. This newly developed method was applied to rat plasma samples after intravenous administration of C60 solubilized with polyvinylpyrrolidone.
采用高效液相色谱法，结合紫外和质谱检测来测定富勒烯（C60）。每种方法的检测限分别为每次进样0.05 ng和2.0 ng（信噪比（S/N = 2））。用固相萃取法提取添加了C60（10微克/毫升）的大鼠血浆，回收率为62.1%，日内测定之间的变异系数（c.v.，n = 5）为4.0%。紫外检测下，峰面积与血浆C60浓度的校准曲线在0.5 - 60微克/毫升范围内显示出良好的线性（r = 0.996）。这种新开发的方法应用于静脉注射用聚乙烯吡咯烷酮增溶的C60后的大鼠血浆样品。
