Ahn M J, Noh Y H, Lee Y S, Lee J H, Chung T J, Kim I S, Choi I Y, Kim S H, Lee J S, Lee K H
Department of Medicine, College of Medicine, Hanyang University, KURI Hospital, Kyomun dong, Kyunggi-do, Korea.
Eur J Cancer. 1997 Jul;33(8):1309-13. doi: 10.1016/s0959-8049(97)00113-5.
In order to assess the role of telomerase in development of malignant gastric cancer, we measured the telomerase activity in gastric cancer tissues and normal tissues obtained from 95 patients by employing recently developed sensitive PCR (polymerase chain reaction)-based telomerase assay (telomeric repeat amplification protocol, TRAP). We also investigated how telomerase activity related to other clinicopathological findings including DNA ploidy and K-RAS gene point mutation. The telomerase activity was present in 85 of the 95 gastric cancer tissues, whereas we detected no telomerase activity in any normal tissue. The incidence of telomerase activity in gastric cancer tissues was not correlated to age, sex, tumour stage, histological grade, DNA ploidy or K-RAS mutation. Disease-free or overall survival of patients having tumours with detectable telomerase activity was not significantly different from that of those without telomerase activity. These findings suggest that telomerase may play a key role in the establishment and progression of the gastric cancer and further studies will be needed to elucidate the biological role of telomerase in gastric cancer.
为了评估端粒酶在恶性胃癌发生发展中的作用，我们采用最近开发的基于聚合酶链反应（PCR）的灵敏端粒酶检测法（端粒重复序列扩增法，TRAP），对95例患者的胃癌组织和正常组织中的端粒酶活性进行了检测。我们还研究了端粒酶活性与其他临床病理特征（包括DNA倍体和K-RAS基因点突变）之间的关系。95例胃癌组织中有85例存在端粒酶活性，而在任何正常组织中均未检测到端粒酶活性。胃癌组织中端粒酶活性的发生率与年龄、性别、肿瘤分期、组织学分级、DNA倍体或K-RAS突变均无相关性。端粒酶活性可检测的肿瘤患者的无病生存期或总生存期与无端粒酶活性的患者无显著差异。这些发现表明，端粒酶可能在胃癌的发生和发展中起关键作用，需要进一步研究以阐明端粒酶在胃癌中的生物学作用。
