Wang Y, Sharma R K, Agarwal A
Department of Urology, Cleveland Clinic Foundation, OH 44195, USA.
Urology. 1997 Sep;50(3):414-7. doi: 10.1016/S0090-4295(97)00217-3.
Although pre-freeze and post-thaw semen quality in patients with cancer is poor, it is not clear whether lipid peroxidation affects semen quality. This study assessed (1) whether poor semen quality in patients with cancer is caused by lipid peroxidation, and (2) whether patient age or sperm motility is associated with lipid peroxidation.
Lipid peroxidation was measured by determining malonaldehyde levels using the thiobarbituric acid method. Malonaldehyde levels were measured in cryopreserved semen specimens from patients with testicular (n = 15) or nontesticular cancer (n = 16) and normal men (control subjects, n = 20). A computer-assisted semen analyzer was used to determine the sperm concentration and motility before and after cryopreservation.
Malonaldehyde levels did not differ in frozen-thawed semen specimens among patients with testicular (25.90 +/- 1.00 nM/10(8) sperm/hr) or nontesticular cancer (24.48 +/- 1.66 nM/10(8) sperm/hr), and control subjects (24.86 +/- 1.43 nM/10(8) sperm/hr). Malonaldehyde levels did not correlate with post-thaw sperm motility or patient age in patients with testicular or nontesticular cancer. Post-thaw sperm motility from patients with testicular or nontesticular cancer was significantly lower than that in normal subjects.
The poor post-thaw semen quality in patients with testicular or nontesticular cancer is not related to lipid peroxidation but may be caused by other factors such as sperm membrane stress induced during the freeze-thaw process.
尽管癌症患者冻融前和冻融后的精液质量较差，但尚不清楚脂质过氧化是否会影响精液质量。本研究评估了：（1）癌症患者精液质量差是否由脂质过氧化引起；（2）患者年龄或精子活力是否与脂质过氧化有关。
采用硫代巴比妥酸法测定丙二醛水平以检测脂质过氧化。对睾丸癌患者（n = 15）、非睾丸癌患者（n = 16）以及正常男性（对照组，n = 20）的冷冻精液标本进行丙二醛水平测定。使用计算机辅助精液分析仪测定冷冻前后的精子浓度和活力。
睾丸癌患者（25.90±1.00 nM/10⁸精子/小时）、非睾丸癌患者（24.48±1.66 nM/10⁸精子/小时）以及对照组（24.86±1.43 nM/10⁸精子/小时）的冻融精液标本中丙二醛水平无差异。睾丸癌或非睾丸癌患者的丙二醛水平与冻融后精子活力或患者年龄均无相关性。睾丸癌或非睾丸癌患者冻融后的精子活力显著低于正常受试者。
睾丸癌或非睾丸癌患者冻融后精液质量差与脂质过氧化无关，可能是由其他因素引起的，如冻融过程中诱导的精子膜应激。
