Fujimura S, Kondo T, Handa M, Saito R, Shiraishi Y, Matsumura Y, Sasaki H, Okada Y, Kumagai M, Nakada T
Rinsho Kyobu Geka. 1989 Feb;9(1):13-8.
We attempted to establish the method of early diagnosis of rejection in lung transplantation using dog, rat and monkey experimental models. Orthotopic left lung allotransplantation was performed in these animals and lymphocytes from peripheral blood (PBL) and/or bronchoalveolar lavage fluid (BALF) were examined as a probe of rejection monitoring. The recipient dog showed a decreased response of PBL to PHA and an increased spontaneous blastogenesis (SB) at the period undergoing rejection which were confirmed by blood samples taken within 2 days before sacrifice of the animal. In rat lung transplantation, 2 groups of animal pair were used; WKA to F344, and WKY to F344. The former recipient rejected the graft within 5 days postoperatively and the latter rejected within 2 weeks after the operation. In both groups parameters for detection of rejection included lymphocyte count, SB and T cell subsets of PBL and BALF lymphocyte. Lymphocyte SB of BALF from lung transplant reflected early stage of rejection and preceded the changes in PBL. This seemed to be useful for early diagnosis of lung allograft rejection. In monkey experiment, BALF lymphocyte from the transplanted lung seemed to well reflect the grade of rejection when compared total cell count and differential cell count with histologic findings of the graft, though the number of this experiment was small.
我们试图利用狗、大鼠和猴子实验模型建立肺移植排斥反应的早期诊断方法。对这些动物进行了原位左肺同种异体移植，并检查外周血淋巴细胞（PBL）和/或支气管肺泡灌洗液（BALF）作为排斥反应监测的指标。接受移植的狗在排斥反应期间PBL对PHA的反应降低，自发增殖（SB）增加，这在动物处死前2天内采集的血样中得到证实。在大鼠肺移植中，使用了2组动物对；WKA对F344，以及WKY对F344。前者的受体在术后5天内排斥移植物，后者在术后2周内排斥。在两组中，检测排斥反应的参数包括淋巴细胞计数、SB以及PBL和BALF淋巴细胞的T细胞亚群。肺移植BALF的淋巴细胞SB反映了排斥反应的早期阶段，且早于PBL的变化。这似乎对肺同种异体移植排斥反应的早期诊断有用。在猴子实验中，尽管该实验的数量较少，但将移植肺的BALF淋巴细胞的总细胞计数和细胞分类计数与移植物的组织学结果进行比较时，似乎能很好地反映排斥反应的程度。
