Saito R, Kondo T, Fujimura S, Handa M, Ichinose T, Shiraishi Y
Department of Surgery, Tohoku University, Sendai.
Tohoku J Exp Med. 1990 Mar;160(3):231-49. doi: 10.1620/tjem.160.231.
Left lung transplantation was performed in two combinations of rat strains. In group 1, lung grafts were rejected within 7 day postoperatively, and in group 2, grafts were rejected within 18 day postoperatively. The histological appearance of rejection was classified into 4 stages, and lymphocytes from peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were collected at each stage. In group 1, a significant decrease in the number of lymphocytes in PB was observed as the rejection progressed, whereas the number of lymphocytes in PB increased slightly in group 2. The number of total cells in BALF increased significantly as the rejection progressed in both groups. A marked increase in the value of spontaneous blastogenesis (SB) was observed in stage 2 in BALF lymphocytes, whereas that in PB lymphocytes was found to increase after stage 3 in both groups. The ratio of T-helper/T-nonhelper did not change significantly in PB lymphocytes in both groups. On the other hand, a significant decrease in the value of T-helper/T-nonhelper (less than 1.0) was observed in BALF at stage 3 and 4 in both groups. These results show that the studies of BALF lymphocytes were very useful for early diagnosis of rejection in lung transplantation.
在两种大鼠品系组合中进行了左肺移植。在第1组中，肺移植在术后7天内被排斥，在第2组中，移植在术后18天内被排斥。排斥反应的组织学表现分为4个阶段，在每个阶段收集外周血（PB）和支气管肺泡灌洗液（BALF）中的淋巴细胞。在第1组中，随着排斥反应的进展，观察到PB中淋巴细胞数量显著减少，而在第2组中PB中淋巴细胞数量略有增加。两组中，随着排斥反应的进展，BALF中总细胞数均显著增加。在BALF淋巴细胞的第2阶段观察到自发增殖（SB）值显著增加，而在两组中PB淋巴细胞的SB值在第3阶段后增加。两组PB淋巴细胞中辅助性T细胞/非辅助性T细胞的比例均无显著变化。另一方面，在两组的第3和第4阶段，BALF中辅助性T细胞/非辅助性T细胞的值显著降低（小于1.0）。这些结果表明，对BALF淋巴细胞的研究对肺移植排斥反应的早期诊断非常有用。
