Katoh S, Mitsui Y, Kitani K, Suzuki T
Radiation Safety Office, University of Tokyo Hospital, Japan.
Neurosci Lett. 1997 Aug 29;232(2):71-4. doi: 10.1016/s0304-3940(97)00582-x.
The rat pheochromocytoma cell line PC12 is useful for studying neuronal cell differentiation since this cell line differentiates into neuron-like cells in response to nerve growth factor (NGF). We demonstrated that PC12h cells, a subclone of PC12 cells, died under hyperoxia (50% O2). This cell death did not occur in the presence of antioxidant reagents. In the dead cells, DNA fragmentation and chromatin condensation were observed, suggesting that hyperoxia-induced apoptosis via reactive oxygen species (ROS). NGF effectively suppressed this hyperoxia-induced apoptosis. Accordingly, the amounts of bcl-2, a proto-oncogene product, increased in the cells rescued from apoptosis by NGF. Furthermore, bcl-2 antisense oligonucleotide canceled this rescuing effect of NGF. The present findings indicate that NGF rescues PC12h cells from hyperoxia-induced apoptosis via up-regulation of bcl-2.
大鼠嗜铬细胞瘤细胞系PC12可用于研究神经元细胞分化，因为该细胞系会在神经生长因子（NGF）的作用下分化为神经元样细胞。我们证明，PC12细胞的亚克隆PC12h细胞在高氧环境（50% O2）下会死亡。在存在抗氧化剂的情况下，这种细胞死亡不会发生。在死亡细胞中，观察到了DNA片段化和染色质浓缩，这表明高氧通过活性氧（ROS）诱导细胞凋亡。NGF可有效抑制这种高氧诱导的细胞凋亡。因此，在被NGF从凋亡中挽救的细胞中，原癌基因产物bcl-2的量增加。此外，bcl-2反义寡核苷酸消除了NGF的这种挽救作用。目前的研究结果表明，NGF通过上调bcl-2使PC12h细胞免受高氧诱导的细胞凋亡。
