Enokido Y, Hatanaka H
Division of Protein Biosynthesis, Osaka University, Japan.
Brain Res. 1990 Dec 17;536(1-2):23-9. doi: 10.1016/0006-8993(90)90004-u.
When clonal rat pheochromocytoma PC12h cells were cultured in a 50% O2 atmosphere, cells gradually died during the cultivation. On the other hand, the addition of NGF at the final concentration of 50 ng/ml could rescue the cells from death. The culture in a 40% O2 atmosphere had little effect on the growth of PC12h cells, as compared with the culture in a normal 20% O2 condition. A very high O2 concentration, as 60%, caused severe damage to PC12h cell growth, and the restoration of cell growth by NGF seemed to be insufficient. PC12h cells were fully differentiated and extended dense long neurites by NGF even in a 50% O2 atmosphere. However, the neurite extension in the culture in a 60% O2 atmosphere was suppressed. The cell-saving effect of NGF on cell death in culture under a 50% O2 atmosphere was dose-dependent, and the ED50 value of NGF was 5 ng/ml. Basic fibroblast growth factor and epidermal growth factor also had a potent effect to rescue the cell death in the high O2 culture, but insulin had no effect. Since the differentiation effects of NGF on PC12h cells are thought to offer a model system to investigate the effect of NGF on neurons, the present observations suggest that a protection machinery for high O2 toxicity to neurons may exist in the neuronal differentiated PC12h cells by NGF, but not in the undifferentiated cells.
当克隆大鼠嗜铬细胞瘤PC12h细胞在50%氧气氛围中培养时，细胞在培养过程中逐渐死亡。另一方面，添加终浓度为50 ng/ml的神经生长因子（NGF）可使细胞免于死亡。与在正常20%氧气条件下培养相比，在40%氧气氛围中培养对PC12h细胞的生长影响不大。60%这样非常高的氧气浓度会对PC12h细胞生长造成严重损害，而且NGF对细胞生长的恢复作用似乎并不充分。即使在50%氧气氛围中，NGF也能使PC12h细胞完全分化并伸出密集的长神经突。然而，在60%氧气氛围中培养时神经突的延伸受到抑制。在50%氧气氛围下培养时，NGF对细胞死亡的挽救作用呈剂量依赖性，NGF的半数有效剂量（ED50）值为5 ng/ml。碱性成纤维细胞生长因子和表皮生长因子对挽救高氧培养中的细胞死亡也有显著作用，但胰岛素没有作用。由于NGF对PC12h细胞的分化作用被认为提供了一个研究NGF对神经元作用的模型系统，目前的观察结果表明，在神经元分化的PC12h细胞中可能存在一种针对高氧对神经元毒性的保护机制，但在未分化细胞中则不存在。
