Radford A D, Bennett M, McArdle F, Dawson S, Turner P C, Glenn M A, Gaskell R M
Department of Veterinary Pathology, University of Liverpool, Neston, S. Wirral, UK.
Vaccine. 1997 Aug-Sep;15(12-13):1451-8. doi: 10.1016/s0264-410x(97)00059-5.
A reverse transcriptase polymerase chain reaction (PCR) was used to amplify a 235 bp hypervariable region of the feline calicivirus (FCV) genome which encodes part of the capsid protein. Sequence from this region was used to compare viruses used in three attenuated vaccines to viruses isolated from vaccinated cats with clinical signs of FCV-infection (vaccine failures). All three vaccine viruses contained sequence similar to that published for FCV strain F9 (Carter et al. 1992, Virology 190, 443-448). However, two of the three vaccines contained a separate sequence which was 20.67% distant (number of nucleotide substitutions per 100 bases) from F9. The sequences derived from isolates obtained from vaccine failures fell into two categories. Most were distinct (21.33-38.00% distant) from vaccine sequence. However, in some cases, sequences were sufficiently similar to the vaccines' (0.00-5.33% distant) to suggest that the isolate may have originated from the vaccine. In addition, comparison of sequence determined for isolates from the same disease outbreak showed them to be closely related (0.00-1.33% distant), whereas epidemiologically unrelated isolates were 20.67-38.00% distant.
采用逆转录聚合酶链反应（PCR）扩增猫杯状病毒（FCV）基因组中一个235 bp的高变区，该区域编码衣壳蛋白的一部分。利用该区域的序列，将三种减毒活疫苗中使用的病毒与从出现FCV感染临床症状的接种猫（疫苗失效）中分离出的病毒进行比较。所有三种疫苗病毒都含有与FCV F9株（Carter等人，1992年，《病毒学》190卷，443 - 448页）公布的序列相似的序列。然而，三种疫苗中的两种含有一个单独的序列，该序列与F9的差异为20.67%（每100个碱基的核苷酸替换数）。从疫苗失效的猫中分离出的病毒序列分为两类。大多数与疫苗序列不同（差异为21.33 - 38.00%）。然而，在某些情况下，序列与疫苗的序列足够相似（差异为0.00 - 5.33%），表明分离出的病毒可能源自疫苗。此外，对同一疾病暴发中分离出的病毒序列进行比较发现，它们密切相关（差异为0.00 - 1.33%），而在流行病学上不相关的分离株差异为20.67 - 38.00%。
