Kattesh H G, Baumbach G A, Gillespie B B, Schneider J F, Murai J T
Department of Animal Science, University of Tennessee, Knoxville 37901-1071, USA.
Biol Neonate. 1997;72(3):192-200. doi: 10.1159/000244484.
Thirty-five time-dated pregnant gilts were used to document plasma levels of total and free cortisol, corticosteroid-binding globulin (CBG) binding capacity, and percent distribution of cortisol among protein-bound (CBG and albumin) and free forms in the fetal pig during the last 24 days of gestation. Plasma from fetal pigs on days 110-114 of gestation (gestation length 114 days) had significantly higher levels of total cortisol (p < 0.01), percent albumin-bound and free cortisol (p < 0.10), and free cortisol concentration (p < 0.05) compared to samples on days 90, 100 and 105. Fetal plasma CBG binding capacity increased (p < 0.05) linearly from day 100 to 114. Fetal pigs located in the cervical region of the uterus had lower (p < 0.05) total and free cortisol and higher (p < 0.05) albumin and total protein concentrations compared to fetuses in the middle and oviductal regions. Total, percent free and free cortisol concentrations in maternal plasma on days 105-114 were greater (p < 0.10) than that measured on days 12-100 of gestation. These results suggest that the developmental patterns of plasma cortisol and CBG in the prenatal pig are directly related and highly similar to those of another precocious species, the sheep.
选用35头定期配种的妊娠母猪，以记录妊娠最后24天胎猪血浆中总皮质醇和游离皮质醇水平、皮质类固醇结合球蛋白（CBG）结合能力，以及皮质醇在蛋白质结合形式（CBG和白蛋白）和游离形式之间的分布百分比。与妊娠90、100和105天采集的样本相比，妊娠110 - 114天（妊娠期长度为114天）的胎猪血浆中总皮质醇水平显著更高（p < 0.01），白蛋白结合皮质醇和游离皮质醇的百分比显著更高（p < 0.10），游离皮质醇浓度显著更高（p < 0.05）。从妊娠100天到114天，胎猪血浆CBG结合能力呈线性增加（p < 0.05）。与子宫中部和输卵管区域的胎儿相比，位于子宫颈部区域的胎猪总皮质醇和游离皮质醇水平更低（p < 0.05），白蛋白和总蛋白浓度更高（p < 0.05）。妊娠105 - 114天母体血浆中的总皮质醇、游离皮质醇百分比和游离皮质醇浓度高于妊娠12 - 100天测得的水平（p < 0.10）。这些结果表明，产前猪血浆皮质醇和CBG的发育模式直接相关，且与另一种早熟物种绵羊的发育模式高度相似。
