Csuka I, Banfalvi G
Institute of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University Medical School, Budapest, Hungary.
DNA Cell Biol. 1997 Aug;16(8):979-84. doi: 10.1089/dna.1997.16.979.
Nascent Hg-DNA synthesized by the incorporation of Hg-dCTP in reversibly permeable cells of murine thymocytes has been characterized earlier. Here we describe the analysis of 5' ends of oligonucleotides isolated from thymocytes 48 hr after a single dose of emetine administration to mice. This small-molecular-weight population of nascent DNA shorter than Okazaki fragments was absent in control cells. More than 90% of the terminally 32P-labeled oligonucleotides carried a terminally phosphorylated RNA moiety at the 5' end, as demonstrated by alkaline hydrolysis. The size of the short nascent DNA fragments carrying RNA primers ranged between 9 and 50 nucleotides with an average chain length of 15 nucleotides. These oligomers are regarded as the precursors of the Okazaki fragments.
此前已对通过在小鼠胸腺细胞的可逆通透细胞中掺入汞脱氧胞苷三磷酸（Hg-dCTP）合成的新生汞-DNA进行了表征。在此，我们描述了对给小鼠单次注射依米丁48小时后从胸腺细胞中分离出的寡核苷酸5'末端的分析。在对照细胞中不存在这种比冈崎片段短的小分子新生DNA群体。如通过碱性水解所证明的，超过90%的末端用32P标记的寡核苷酸在5'末端带有末端磷酸化的RNA部分。携带RNA引物的短新生DNA片段的大小在9至50个核苷酸之间，平均链长为15个核苷酸。这些寡聚物被视为冈崎片段的前体。
