Minchinton R M, Noonan K, Johnson T J
Australian Red Cross Blood Service, Queensland, Brisbane, Australia.
Vox Sang. 1997;73(2):87-92. doi: 10.1046/j.1423-0410.1997.7320087.x.
This study was an attempt to improve detection and characterization of alloantibodies to neutrophil antigens, using the monoclonal antibody immobilisation of granulocyte antigens (MAIGA) assay.
We explored the effect of different detergent concentrations, detergent class, stabilising additives, combinations of detergents, and use of a broad range of monoclonal antibodies on the results of the MAIGA assay.
Non-ionic detergents, Triton X and Nonidet, were confirmed as the most suitable for solubilisation of the recognised, neutrophil-associated alloantigens. Anionic and zwitterionic detergents did not assist the demonstration and characterisation of new neutrophil alloantigens in the MAIGA assay. Some reproducible positive MAIGA assay results were obtained for antisera 01, 08, anti-RED, anti-NB2, and anti-NB1, using particular monoclonal antibodies.
Although we are reluctant to suggest association of the targeted antigens with the particular glycoproteins identified by the capture monoclonal antibodies, NB1 glycoprotein may be closely associated, before or after antibody binding, with CD46, CD11b, CD50, and/or CD66b.
本研究旨在通过粒细胞抗原单克隆抗体固定法（MAIGA）检测，改进对中性粒细胞抗原同种抗体的检测与特性鉴定。
我们探讨了不同去污剂浓度、去污剂种类、稳定剂添加剂、去污剂组合以及多种单克隆抗体的使用对MAIGA检测结果的影响。
非离子去污剂Triton X和Nonidet被确认为最适合溶解已识别的中性粒细胞相关同种抗原。在MAIGA检测中，阴离子和两性离子去污剂无助于新的中性粒细胞同种抗原的显示与特性鉴定。使用特定单克隆抗体时，抗血清01、08、抗RED、抗NB2和抗NB1获得了一些可重复的MAIGA检测阳性结果。
尽管我们不愿表明靶向抗原与捕获单克隆抗体所识别的特定糖蛋白之间存在关联，但NB1糖蛋白在抗体结合之前或之后可能与CD46、CD11b、CD50和/或CD66b密切相关。
