Stroncek D F, Shankar R A, Plachta L B, Clay M E, Dalmasso A P
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Transfusion. 1993 May;33(5):399-404. doi: 10.1046/j.1537-2995.1993.33593255600.x.
The neutrophil-specific NB antigen system has been serologically characterized with human alloantisera. Two alleles, NB1 and NB2, have been described; however, there may be important quantitative or qualitative variation in the expression of NB1 and NB2. Human alloantibodies have been used to identify the 58- to 64-kDa glycoprotein (GP) on which NB1 antigen is located, but an NB2 antigen-bearing molecule has not yet been identified. To identify the NB2 molecule, human alloantibody to NB1 was used to isolate the 58- to 64-kDa NB1 GP, and rabbits were immunized with this GP. Two rabbit antisera were produced. Both antisera immunoblotted and immunoprecipitated the 58- to 64-kDa GP on which NB1 is located, but neither identified the molecule on which NB2 is located. The inability of two rabbit polyclonal antibodies specific for the NB1 molecule to react with the NB2-bearing molecule suggests that considerable differences may exist between these two molecules or that NB2 as currently defined is not related to NB1.
中性粒细胞特异性NB抗原系统已通过人同种异体抗血清进行了血清学鉴定。已描述了两个等位基因，NB1和NB2；然而，NB1和NB2的表达可能存在重要的数量或质量差异。人同种异体抗体已被用于鉴定NB1抗原所在的58至64 kDa糖蛋白（GP），但尚未鉴定出携带NB2抗原的分子。为了鉴定NB2分子，使用针对NB1的人同种异体抗体分离出58至64 kDa的NB1 GP，并以此GP免疫兔子。产生了两种兔抗血清。两种抗血清均通过免疫印迹和免疫沉淀鉴定出NB1所在的58至64 kDa GP，但均未鉴定出NB2所在的分子。两种对NB1分子具有特异性的兔多克隆抗体无法与携带NB2的分子发生反应，这表明这两种分子之间可能存在相当大的差异，或者目前定义的NB2与NB1无关。
