Cestèle S, Gordon D, Kopeyan C, Rochat H
Laboratoire de Biochimie, Impénierie des Protéines UNR6560, Université de la Méditeranneé CNRS IFR Jean Roche, Faculté de Médecine Nord Bd Pierre Dramard, Marseille, France.
Insect Biochem Mol Biol. 1997 Jun;27(6):523-8. doi: 10.1016/s0965-1748(97)00027-1.
Scorpion toxin Lqq III binds to a single class of high affinity (Kd = 72 +/- 19 pM) and low capacity (Bmax = 2.5 +/- 0.2 pmol/mg) binding sites in cockroach neuronal membranes. Its binding was inhibited by Lqh alpha IT (IC50 = 80 +/- 30 pM) and sea-anemone toxin ATX II (IC50 = 2.5 +/- 0.3 nM), suggesting that Lqq III is a specific probe for receptor site 3 on cockroach sodium channels. This was confirmed by competitive binding experiments between 125I-Lqq III and scorpion alpha-toxins which have less toxicity in insects.
蝎毒素Lqq III与蟑螂神经元膜上一类高亲和力（解离常数Kd = 72 ± 19皮摩尔）和低容量（最大结合量Bmax = 2.5 ± 0.2皮摩尔/毫克）的结合位点结合。其结合受到LqhαIT（半数抑制浓度IC50 = 80 ± 30皮摩尔）和海葵毒素ATX II（IC50 = 2.5 ± 0.3纳摩尔）的抑制，这表明Lqq III是蟑螂钠通道受体位点3的特异性探针。125I-Lqq III与对昆虫毒性较小的蝎α毒素之间的竞争性结合实验证实了这一点。
