Bañuls A L, Guerrini F, Le Pont F, Barrera C, Espinel I, Guderian R, Echeverria R, Tibayrenc M
Centre d'Etudes sur le Polymorphisme des Microorganismes (CEPM), Centre National de la Recherche Scientifique (CNRS)/Institut Français de Recherche Scientifique pour le Développement en Coopération (ORSTOM), Montpellier, France.
J Eukaryot Microbiol. 1997 Sep-Oct;44(5):408-11. doi: 10.1111/j.1550-7408.1997.tb05716.x.
The taxonomic attribution of four Leishmania stocks isolated from humans in Ecuador has been explored by both multilocus enzyme electrophoresis and random amplified polymorphic DNA. For three loci, MLEE results showed patterns suggesting a heterozygous state for a diploid organism, while the corresponding homozygous states are characteristic of the Leishmania panamensis/guyanensis complex and Leishmania braziliensis, respectively. Other enzyme loci showed characters attributable to either the L. panamensis/ guyanensis complex or L. braziliensis. RAPD profiles exhibited for several primers a combination of the Leishmania panamensis/ guyanensis complex and L. braziliensis characters. These data hence suggest that the four stocks are the result of hybridization between L. panamensis/guyanensis and L. braziliensis. MLEE data show that the results cannot be attributed to either mixture of stocks, or an F1 in the framework of a simple Mendelian inheritance.
通过多位点酶电泳和随机扩增多态性DNA技术，对从厄瓜多尔人体分离出的4株利什曼原虫株的分类归属进行了研究。对于三个基因座，多位点酶电泳结果显示出的模式表明该二倍体生物处于杂合状态，而相应的纯合状态分别是巴拿马利什曼原虫/圭亚那利什曼原虫复合体和巴西利什曼原虫的特征。其他酶基因座显示出可归因于巴拿马利什曼原虫/圭亚那利什曼原虫复合体或巴西利什曼原虫的特征。几种引物的随机扩增多态性DNA图谱呈现出巴拿马利什曼原虫/圭亚那利什曼原虫复合体和巴西利什曼原虫特征的组合。因此，这些数据表明这4个虫株是巴拿马利什曼原虫/圭亚那利什曼原虫和巴西利什曼原虫杂交的结果。多位点酶电泳数据表明，结果不能归因于虫株的混合，也不能归因于简单孟德尔遗传框架下的F1代。
