Dujardin J C, Bañuls A L, Llanos-Cuentas A, Alvarez E, DeDoncker S, Jacquet D, Le Ray D, Arevalo J, Tibayrenc M
Laboratory of Protozoology, Institute of Tropical Medicine, "Prince Leopold", Antwerp, Belgium.
Acta Trop. 1995 Aug;59(4):293-307. doi: 10.1016/0001-706x(95)00094-u.
During an outbreak of tegumentary leishmaniasis that developed in the 1990s in the Eastern Andean valley of Huanuco, Peru, the coexistence of Andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. Seven human isolates of Leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic DNA and molecular karyotyping. The three methods identified 3 isolates as L. braziliensis, and 4 isolates as putative hybrids with characters of L. braziliensis and L. peruviana. Data from Huanuco are compared to previous results from other areas endemic for uta. Biological and epidemiological implications are discussed.
在20世纪90年代秘鲁瓦努科东安第斯山谷爆发皮肤利什曼病期间，出于生态和地理原因，怀疑存在安第斯型（乌塔病）和丛林利什曼病的共存情况，并进行了同域采样。通过多位点酶电泳、随机扩增多态性DNA和分子核型分析对7株利什曼原虫人分离株进行了鉴定。这三种方法将3株分离株鉴定为巴西利什曼原虫，4株分离株鉴定为具有巴西利什曼原虫和秘鲁利什曼原虫特征的推定杂交种。将瓦努科的数据与乌塔病其他流行地区的先前结果进行了比较。讨论了生物学和流行病学意义。
