利用野油菜黄单胞菌野油菜致病变种的recA突变体评估recA蛋白在植物致病中的作用。

Evaluation of the role of recA protein in plant virulence with recA mutants of Xanthomonas campestris pv. campestris.

作者信息

Martínez S, Martínez-Salazar J, Camas A, Sánchez R, Soberón-Chávez G

机构信息

Departamento de Microbiologia Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.

出版信息

Mol Plant Microbe Interact. 1997 Sep;10(7):911-6. doi: 10.1094/MPMI.1997.10.7.911.

DOI:10.1094/MPMI.1997.10.7.911

PMID:9304862

Abstract

Xanthomonas campestris pv. campestris NRRL B1459 recA mutants were isolated by recombination with an interrupted Rhizobium etli recA gene and selection of double recombinants. The mutants were impaired in homologous genetic recombination and in DNA repair as judged by their sensitivity to methyl-methane-sulfonate and to UV irradiation; these defects are complemented in trans by the R. etli recA gene. Virulence of X. campestris pv. campestris NRRL B1459 to cabbage is considerably diminished by the recA mutation. The recA mutation is not correlated with the frequency of occurrence of a genetic rearrangement that affects chemotaxis, plant virulence, and xanthan gum production.

摘要

通过与中断的费氏中华根瘤菌recA基因进行重组并筛选双重组体，分离出野油菜黄单胞菌野油菜致病变种NRRL B1459的recA突变体。根据它们对甲磺酸甲酯和紫外线照射的敏感性判断，这些突变体在同源基因重组和DNA修复方面存在缺陷；这些缺陷可被费氏中华根瘤菌recA基因反式互补。recA突变使野油菜黄单胞菌野油菜致病变种NRRL B1459对甘蓝的毒力显著降低。recA突变与影响趋化性、植物毒力和黄原胶产生的基因重排的发生频率无关。