Martínez-Salazar J M, Palacios A N, Sánchez R, Caro A D, Soberón-Chavez G
Departamento de Bioingeniería, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Mol Microbiol. 1993 Jun;8(6):1053-61. doi: 10.1111/j.1365-2958.1993.tb01650.x.
A transposon (Tn5-SC) was constructed that can be used to quantify genetic deletions or amplifications. This transposon was used to evaluate the genomic stability of Xanthomonas campestris pv. campestris NRRL B1459 and we found that the genome of this bacterium is as stable as other Gram-negative bacteria or even more stable. Homologous recombination between plasmid sequences was determined in strain NRRL B1459 and was found to occur at a similar level to that reported for other Gram-negative bacteria. We report here that in X.c.c. NRRL B1459 there is no straightforward correlation between the occurrence of genetic rearrangements and frequency of homologous recombination. These data are discussed with respect to the reported instability of strain NRRL B1459 for xanthan gum production.
构建了一种可用于量化基因缺失或扩增的转座子（Tn5-SC）。该转座子用于评估野油菜黄单胞菌野油菜致病变种NRRL B1459的基因组稳定性，我们发现该细菌的基因组与其他革兰氏阴性菌一样稳定，甚至更稳定。在菌株NRRL B1459中测定了质粒序列之间的同源重组，发现其发生水平与其他革兰氏阴性菌报道的相似。我们在此报告，在野油菜黄单胞菌野油菜致病变种NRRL B1459中，基因重排的发生与同源重组频率之间没有直接相关性。结合已报道的NRRL B1459菌株在生产黄原胶方面的不稳定性对这些数据进行了讨论。
