Garcia-Palmer F J, Pericas J, Matamala J C, Puigserver P, Bonet M L, Palou A, Gianotti M
Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Biochem Mol Biol Int. 1997 Sep;42(6):1151-61. doi: 10.1080/15216549700203621.
The aim of the present work is to investigate the effect of starvation on brown adipose tissue thermogenic activity with aging. Interscapular brown adipose tissue from female Wistar rats of different ages was used; half of them were fed and the other half were starved for 24 hours. Mitochondria were isolated and mitochondrial protein content, GDP-binding, Cytochrome-c Oxidase activity and uncoupling protein levels were measured. Results show a decrease of all studied parameters, indicating a diminished thermogenic activity with age. The response to starvation is almost the same in all the parameters studied: a general reduction with starvation and a progressive disappearance of this response to starvation with aging. On the whole, these results would indicate a deficient regulation of brown adipose tissue thermogenic activity in old animals, as it happens in other animal models with an alterated thermogenesis.
本研究的目的是探究饥饿对棕色脂肪组织产热活性随衰老变化的影响。使用来自不同年龄雌性Wistar大鼠的肩胛间棕色脂肪组织；其中一半喂食，另一半饥饿24小时。分离线粒体并测量线粒体蛋白含量、GDP结合、细胞色素c氧化酶活性和解偶联蛋白水平。结果显示所有研究参数均下降，表明产热活性随年龄降低。在所有研究参数中，对饥饿的反应几乎相同：饥饿时普遍降低，且随着衰老，这种对饥饿的反应逐渐消失。总体而言，这些结果表明老年动物棕色脂肪组织产热活性调节不足，就像在其他产热改变的动物模型中一样。
