氯胺酮抑制脂多糖处理的大鼠肺泡巨噬细胞中的一氧化氮合酶。

Ketamine inhibits nitric oxide synthase in lipopolysaccharide-treated rat alveolar macrophages.

作者信息

Li C Y, Chou T C, Wong C S, Ho S T, Wu C C, Yen M H, Ding Y A

机构信息

Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.

出版信息

Can J Anaesth. 1997 Sep;44(9):989-95. doi: 10.1007/BF03011971.

DOI:10.1007/BF03011971

PMID:9305563

Abstract

PURPOSE

To evaluate the effects of ketamine on the activity and protein expression of inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide (LPS) in rat alveolar macrophages.

METHODS

Pulmonary alveolar macrophages isolated from Wistar-Kyoto rats were used. After incubation of macrophages with ketamine (1, 10, or 100 microM) and LPS (1 microgram.ml-1) for 24 hr, the cell-free medium was removed for measuring the nitrite and tumour necrosis factor-alpha (TNF-alpha) levels by Griess reaction and ELISA kit, respectively. The harvested macrophages were also used to determine the activity of iNOS by using the conversion of [3H]-L-arginine to [3H]-L-citrulline method. In addition, the protein expression of iNOS was detected by Western blot analysis.

RESULTS

In rat alveolar macrophages, (i) ketamine (1 to 100 microM) caused a dose-dependent suppression of the production of nitrite and TNF-alpha induced by LPS and (ii) ketamine (100 microM) inhibited the activity (46.5 +/- 4.8%, P < 0.05) and protein expression (35 +/- 11%, P < 0.05) of iNOS in response to LPS.

CONCLUSION

These results show that ketamine inhibits the activity and expression of iNOS in LPS-activated alveolar macrophages, which may be associated with the reduction of the release of TNF-alpha following LPS treatment.

摘要

目的

评估氯胺酮对脂多糖（LPS）诱导的大鼠肺泡巨噬细胞中诱导型一氧化氮合酶（iNOS）活性及蛋白表达的影响。

方法

使用从Wistar - Kyoto大鼠分离的肺泡巨噬细胞。巨噬细胞与氯胺酮（1、10或100微摩尔）和LPS（1微克·毫升⁻¹）孵育24小时后，去除无细胞培养基，分别通过格里斯反应和ELISA试剂盒测定亚硝酸盐和肿瘤坏死因子-α（TNF-α）水平。收获的巨噬细胞还用于通过[³H]-L-精氨酸转化为[³H]-L-瓜氨酸的方法测定iNOS的活性。此外，通过蛋白质印迹分析检测iNOS的蛋白表达。