福氏志贺菌特定型抗原V：温和噬菌体SfV葡糖基转移酶基因的克隆、测序及特性分析

Shigella flexneri type-specific antigen V: cloning, sequencing and characterization of the glucosyl transferase gene of temperate bacteriophage SfV.

作者信息

Huan P T, Whittle B L, Bastin D A, Lindberg A A, Verma N K

机构信息

Division of Biochemistry and Molecular Biology, School of Life Sciences, The Australian National University, Canberra.

出版信息

Gene. 1997 Aug 22;195(2):207-16. doi: 10.1016/s0378-1119(97)00144-3.

DOI:10.1016/s0378-1119(97)00144-3

PMID:9305766

Abstract

With lysogeny by bacteriophage SfV, Shigella flexneri serotype Y is converted to serotype 5a. The glucosyl transferase gene (gtr) from bacteriophage SfV of S. flexneri, involved in serotype-specific conversion, was cloned and characterized. The DNA sequence of a 3.7 kb EcoRI-BamHI fragment of bacteriophage SfV which includes the gtr gene was determined. This gene, encoding a polypeptide of 417 aa with 47.67 kDa molecular mass, caused partial serotype conversion of S. flexneri from serotype Y to type V antigen as demonstrated by Western blotting and the sensitivity of the hybrid strain to phage Sf6. The deduced protein of the partially sequenced open reading frame upstream of the gtr showed similarity to various glycosyl transferases of other bacteria. Orf3, separated from the gtr by a non-coding region and transcribed convergently, codes for a 167 aa (18.8 kDa) protein found to have homology with tail fibre genes of phage lambda and P2.

摘要

通过噬菌体SfV的溶原化作用，弗氏志贺氏菌Y血清型可转变为5a血清型。参与血清型特异性转变的弗氏志贺氏菌噬菌体SfV的葡糖基转移酶基因（gtr）被克隆并进行了表征。测定了噬菌体SfV包含gtr基因的3.7 kb EcoRI - BamHI片段的DNA序列。该基因编码一个由417个氨基酸组成、分子量为47.67 kDa的多肽，如蛋白质免疫印迹法及杂交菌株对噬菌体Sf6的敏感性所示，该基因导致弗氏志贺氏菌从Y血清型部分转变为V型抗原。gtr上游部分测序的开放阅读框所推导的蛋白质与其他细菌的各种糖基转移酶具有相似性。Orf3与gtr由一个非编码区隔开且转录方向相反，编码一种167个氨基酸（18.8 kDa）的蛋白质，发现其与噬菌体λ和P2的尾丝基因具有同源性。