Koide N, Koike S, Adachi W, Amano J, Usuda N, Nagata T
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Surg Today. 1997;27(8):685-91. doi: 10.1007/BF02384977.
In the present study, the expression of bcl-2 protein in esophageal squamous cell carcinoma (SCC) and basaloid carcinoma (BC) was immunohistochemically examined, and its relation to tumor progression and postoperative survival was determined in SCC. A total of 42 SCC and 4 BC tumor samples were fixed with formalin, embedded in paraffin, and stained using monoclonal bcl-2 protein antibody, clone 124. Immunoreactivity was semiquantitatively scored, and the staining results were compared with the pathologic features and survival rates. The cytoplasm of basal cells from the normal esophageal epithelium was stained. In some well- and moderately differentiated SCCs, bcl-2 protein-positive reaction was observed in the peripheral part of the tumor cord, but in poorly differentiated SCC, the cells were weakly or hardly stained. However, in BC, the cells were strongly stained. The immunoreactivity was positive in 45.2% of the SCCs and all of the BCs. There were no significant differences in pathological features or patient survival between the bcl-2 protein-positive and protein-negative SCCs. In conclusion, the expression was not related to tumor progression and had no prognostic significance in SCC. Conversely, BC had strong immunohistochemical expression, probably associated with the differentiation of carcinoma cells simulating the basal cells of the esophagus.
在本研究中，采用免疫组织化学方法检测了食管鳞状细胞癌（SCC）和基底样癌（BC）中bcl-2蛋白的表达，并在SCC中确定了其与肿瘤进展及术后生存的关系。总共42例SCC和4例BC肿瘤样本用福尔马林固定，石蜡包埋，并用克隆号为124的抗bcl-2蛋白单克隆抗体染色。对免疫反应性进行半定量评分，并将染色结果与病理特征和生存率进行比较。正常食管上皮基底细胞的胞质呈阳性染色。在一些高分化和中分化SCC中，在肿瘤索周边部分观察到bcl-2蛋白阳性反应，但在低分化SCC中，细胞呈弱阳性或几乎不着色。然而，在BC中，细胞呈强阳性染色。45.2%的SCC和所有BC的免疫反应性呈阳性。bcl-2蛋白阳性和阴性的SCC在病理特征或患者生存方面无显著差异。总之，在SCC中，该表达与肿瘤进展无关且无预后意义。相反，BC具有强烈的免疫组化表达，可能与癌细胞向食管基底细胞的分化有关。
