Nishida E, Yamanouchi J, Ogata S, Doi K, Itagaki S
Laboratory of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Int J Exp Pathol. 1997 Jun;78(3):169-76. doi: 10.1046/j.1365-2613.1997.240354.x.
Syrian hamsters of the APA strain (APA hamsters) develop spontaneous mesangial thickening in the renal glomeruli from an early age. They also develop focal and segmental glomerulosclerosis (FSG) at and after 6 months of age. In this study, histopathological, immunohistochemical and lectin histochemical examinations were conducted to clarify the modification of the spontaneous renal lesions of APA hamsters by streptozotocin(SZ)-induced diabetes. Histopathological analysis revealed that the expansion of the mesangial region was more prominent and the thickening of the glomerular basement membrane (GBM) was weaker in SZ-treated animals than in non-treated ones. Immunohistochemical analysis suggested that type IV collagen and laminin were involved in the expansion of the mesangial region and thickening of the GBM. In lectin histochemical analysis, podocytes, capillary endothelial cells, GBM and a part of mesangial region of SZ-treated animals were positive for RCA120 and GSL-I with neuraminidase-pretreatment although they were negative for these lectins in non-treated animals. These results suggest that the spontaneous glomerular lesion of APA hamsters is modified qualitatively and quantitatively by SZ-induced diabetes.
APA品系叙利亚仓鼠（APA仓鼠）自幼就会在肾小球中出现自发性系膜增厚。它们在6月龄及之后还会出现局灶节段性肾小球硬化（FSG）。在本研究中，进行了组织病理学、免疫组织化学和凝集素组织化学检查，以阐明链脲佐菌素（SZ）诱导的糖尿病对APA仓鼠自发性肾脏病变的影响。组织病理学分析显示，与未处理动物相比，SZ处理动物的系膜区扩张更明显，肾小球基底膜（GBM）增厚更弱。免疫组织化学分析表明，IV型胶原和层粘连蛋白与系膜区扩张和GBM增厚有关。在凝集素组织化学分析中，经神经氨酸酶预处理后，SZ处理动物的足细胞、毛细血管内皮细胞、GBM和部分系膜区对RCA120和GSL-I呈阳性，而未处理动物对这些凝集素呈阴性。这些结果表明，SZ诱导的糖尿病在质量和数量上改变了APA仓鼠的自发性肾小球病变。
