Oliveira D T, Odell E W
Department of Pathology, School of Dentistry of Bauru, University of Sao Paulo, Brazil.
Oral Oncol. 1997 Jul;33(4):260-2. doi: 10.1016/s0964-1955(97)00081-x.
Altered expression of CD44H an CD44 splice variants is associated with metastasis in several malignancies but its analysis requires knowledge of the normal pattern of expression which is tissue specific. There are considerable regional variations in epithelial differentiation within the mouth, including differences in cell surface glycoproteins. The aim of this study was to determine whether there are regional variations in the expression of CD44 variants in oral epithelia. Frozen and formalin-fixed paraffin-embedded sections of lip vermilion border, buccal mucosa, dorsum and ventrum of tongue, floor of mouth, gingiva and hard palate were stained immunohistochemically for CD44H and the produce of variant exons v3, v4/5, v6 and v9. Paraffin sections were subjected to microwave antigen retrieval. All epithelia stained strongly for all variants in basal, suprabasal and prickle cells and cornified and surface layers and the basal surface of basal cells were negative. Patterns of staining were identical in frozen and formalin-fixed tissue. Despite the structural heterogeneity within oral epithelium, no regional variation was detected.
CD44H及CD44剪接变体的表达改变与多种恶性肿瘤的转移相关，但其分析需要了解具有组织特异性的正常表达模式。口腔内上皮分化存在相当大的区域差异，包括细胞表面糖蛋白的差异。本研究的目的是确定口腔上皮中CD44变体的表达是否存在区域差异。对唇红缘、颊黏膜、舌背和舌腹、口底、牙龈和硬腭的冰冻及福尔马林固定石蜡包埋切片进行免疫组织化学染色，检测CD44H以及可变外显子v3、v4/5、v6和v9的产物。石蜡切片进行微波抗原修复。所有上皮在基底细胞、基底上层细胞、棘细胞、角化层和表层中所有变体均呈强染色，基底细胞的基底表面呈阴性。冰冻组织和福尔马林固定组织的染色模式相同。尽管口腔上皮结构存在异质性，但未检测到区域差异。
