Chan B P, Chan K M, Maffulli N, Webb S, Lee K K
Hong Kong Centre of Sports Medicine and Sports Science, Department of Orthopaedics and Traumatology, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong.
Clin Orthop Relat Res. 1997 Sep(342):239-47.
The effect of basic fibroblast growth factor on the proliferative and chemotactic response of cultured rat patellar tendon fibroblasts was studied in an in vitro wound closure model. In quiescent confluent fibroblast culture, a uniform cell free zone, or wound, was generated mechanically as an in vitro wound. The width of the cell free zone was measured at 0, 6, 12, and 24 hours after the injury, in the presence of 0, 2, 10, or 50 mg/mL of basic fibroblast growth factor. Basic fibroblast growth factor, at a concentration of 10 ng/mL, significantly accelerated wound closure, resulting in almost complete closure by 24 hours after the injury. Basic fibroblast growth factor, at a concentration of 2 ng/mL, significantly enhanced cell proliferation as estimated by 5-Bromo-2'-deoxyuridine incorporation, but increasing the concentration of the growth factor to 50 ng/mL did not show additional improvement. Thus, the enhancement of wound closure by basic fibroblast growth factor may be caused by the cell proliferative response, rather than by chemotaxis.
在体外伤口闭合模型中研究了碱性成纤维细胞生长因子对培养的大鼠髌腱成纤维细胞增殖和趋化反应的影响。在静止的汇合成纤维细胞培养物中，通过机械方式产生一个均匀的无细胞区，即伤口，作为体外伤口。在损伤后0、6、12和24小时，在存在0、2、10或50 mg/mL碱性成纤维细胞生长因子的情况下，测量无细胞区的宽度。浓度为10 ng/mL的碱性成纤维细胞生长因子显著加速了伤口闭合，在损伤后24小时几乎完全闭合。浓度为2 ng/mL的碱性成纤维细胞生长因子通过5-溴-2'-脱氧尿苷掺入法估计显著增强了细胞增殖，但将生长因子浓度增加到50 ng/mL并未显示出进一步改善。因此，碱性成纤维细胞生长因子对伤口闭合的增强作用可能是由细胞增殖反应引起的，而不是趋化作用。
