Reynolds J D, Penning D H, Kimura K A, Dexter F, Henderson J L, Atkins B, Poduska D, Brien J F
Department of Anesthesia, University of Iowa College of Medicine, Iowa City, USA.
Alcohol Clin Exp Res. 1997 Sep;21(6):997-1004.
Ethanol-induced changes in fetal prostaglandin E (PGE) concentration may play a role in the toxic effects of prenatal ethanol exposure. Using the novel technique of in utero microdialysis, the present study tested the hypothesis that acute ethanol exposure changes PGE concentration in the intact cerebral cortex of preterm (93 +/- 1 days of gestation) and near-term (124 +/- 1 days of gestation; term, approximately 147 days) fetal sheep. Fetal sheep were surgically instrumented with a microdialysis probe placed in the parasagittal parietal cortex. Three days later, the effects of maternal infusion of 1 g of ethanol/kg maternal body weight on preterm (n = 6) and near-term (n = 7) fetal cerebral cortical and plasma PGE concentrations were determined. In the preterm fetal cerebral cortex, PGE concentration was increased after ethanol infusion in all six animals studied. The median peak increase was 160% with a 95% confidence interval of 115 to 784%. There was considerable variation in the time of occurrence, magnitude, and duration of this increase. In the near-term fetal cerebral cortex, an increase in PGE concentration was observed after ethanol infusion in 5 of the 7 animals studied, whereas a decrease in PGE concentration was observed in the other two animals. Overall, ethanol did not increase significantly near-term fetal cerebral cortical PGE concentration. For both age groups, ethanol infusion had no effect on fetal plasma PGE concentration. These data indicate that ethanol can affect PGE production in the fetal cerebral cortex and that this effect seems to be gestational-age-dependent.
乙醇诱导的胎儿前列腺素E（PGE）浓度变化可能在产前乙醇暴露的毒性作用中发挥作用。本研究采用子宫内微透析新技术，检验了急性乙醇暴露会改变早产（妊娠93±1天）和近足月（妊娠124±1天；足月约为147天）胎羊完整大脑皮质中PGE浓度这一假设。通过手术给胎羊植入一个置于矢状旁顶叶皮质的微透析探针。三天后，测定母体输注1 g乙醇/ kg母体体重对早产（n = 6）和近足月（n = 7）胎羊大脑皮质及血浆PGE浓度的影响。在早产胎羊的大脑皮质中，所研究的6只动物在乙醇输注后PGE浓度均升高。中位数峰值增加为160%，95%置信区间为115%至784%。这种增加在发生时间、幅度和持续时间上存在相当大的差异。在近足月胎羊的大脑皮质中，所研究的7只动物中有5只在乙醇输注后PGE浓度升高，而另外两只动物中观察到PGE浓度降低。总体而言，乙醇并未显著增加近足月胎羊大脑皮质的PGE浓度。对于两个年龄组，乙醇输注对胎羊血浆PGE浓度均无影响。这些数据表明，乙醇可影响胎羊大脑皮质中PGE的产生，且这种影响似乎与胎龄有关。
