Suwalsky M, Benites M, Villena F, Aguilar F, Sotomayor C P
Faculty of Chemical Sciences, University of Concepción, Chile.
Z Naturforsch C J Biosci. 1997 Jul-Aug;52(7-8):450-8. doi: 10.1515/znc-1997-7-806.
Dieldrin is an organochlorine insecticide highly toxic for human beings. Although its exact mechanism of action is not well known, there is evidence that it acts at the cell membrane level. In fact, the lipophilicity of the pesticide as well as that of the phospholipid bilayer present in biological membranes makes the latter a most likely target for the interaction of dieldrin with living organisms. In order to evaluate its perturbing effect upon cell membranes the pesticide was made to interact with human erythrocytes and molecular models. These studies were performed by scanning electron microscopy on erythrocytes, fluorescence spectroscopy on dimyristoylphosphatidylcholine (DMPC) large unilamellar vesicles and X-ray diffraction on multilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE). It was observed that dieldrin particularly interacted with DMPC liposomes and multilayers perturbing its molecular arrangements. However, no effect was noticed on erythrocytes, which might be due to its high cholesterol content.
狄氏剂是一种对人类剧毒的有机氯杀虫剂。尽管其确切作用机制尚不清楚，但有证据表明它作用于细胞膜水平。事实上，该杀虫剂的亲脂性以及生物膜中存在的磷脂双分子层的亲脂性，使得后者成为狄氏剂与生物体相互作用最可能的靶点。为了评估其对细胞膜的干扰作用，使该杀虫剂与人类红细胞和分子模型相互作用。这些研究通过对红细胞进行扫描电子显微镜观察、对二肉豆蔻酰磷脂酰胆碱（DMPC）大单层囊泡进行荧光光谱分析以及对二肉豆蔻酰磷脂酰胆碱（DMPC）和二肉豆蔻酰磷脂酰乙醇胺（DMPE）多层膜进行X射线衍射分析来进行。观察到狄氏剂特别与DMPC脂质体和多层膜相互作用，扰乱其分子排列。然而，未观察到对红细胞有影响，这可能是由于其高胆固醇含量所致。
