Karasawa T, Maegawa T, Nojiri T, Yamakawa K, Nakamura S
Department of Bacteriology, School of Medicine, Kanazawa University, Ishikawa, Japan.
Microbiol Immunol. 1997;41(8):581-5. doi: 10.1111/j.1348-0421.1997.tb01895.x.
Twenty strains of Clostridium difficile were examined for the effect of arginine on toxin production in a defined medium. In three strains, the production of toxins A and B was greatly enhanced in the absence of arginine. These strains showed distinctively poorer growth in the absence of arginine in comparison with the remaining 17 strains, indicating that the presence of arginine is required for good growth among the three strains. From the present results, test strains were divided into two groups: a group in which arginine insufficiency caused distinctly poor growth and enhanced toxin production, and another group in which there was neither distinctly poor growth nor enhanced toxin production. The phenomenon is discussed in relation to the biosynthesis and catabolism of arginine.
检测了20株艰难梭菌在特定培养基中精氨酸对毒素产生的影响。在三株菌株中，精氨酸缺乏时毒素A和B的产生显著增强。与其余17株菌株相比，这三株菌株在精氨酸缺乏时生长明显较差，表明这三株菌株良好生长需要精氨酸的存在。根据目前的结果，测试菌株被分为两组：一组中精氨酸不足导致生长明显不良且毒素产生增加，另一组中既没有明显的生长不良也没有毒素产生增加。结合精氨酸的生物合成和分解代谢对该现象进行了讨论。
