通过对ospA和ospB基因的序列分析对从日本北海道莱姆病患者中分离出的伽氏疏螺旋体进行鉴定
Characterization of Borrelia garinii isolated from Lyme disease patients in Hokkaido, Japan, by sequence analysis of OspA and OspB genes.
作者信息
Wang J, Masuzawa T, Yanagihara Y
机构信息
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
出版信息
FEMS Microbiol Lett. 1997 Sep 15;154(2):371-5. doi: 10.1111/j.1574-6968.1997.tb12669.x.
The outer surface proteins OspA and OspB genes of clinical Borrelia garinii isolates (JEM1-8) from Hokkaido, Japan were sequenced. One strain, JEM4, has a single ospA gene which is similar to European B. garinii strains PBr (sequence homology value: 94.1%) and T25 (91.2%). Five of the other seven strains exhibit a homologous C-terminus (300 bp) on both ospA and ospB genes (88.7-97.3%). The other two strains seem to be derived from the five strains by ospA or ospB alterations. In a phylogenetic analysis based on ospA, these strains could be classified into B. garinii, but formed independent branches and separated from the typical B. garinii isolates from Europe and Russia.
对来自日本北海道的临床加氏疏螺旋体分离株（JEM1 - 8）的外表面蛋白OspA和OspB基因进行了测序。其中一个菌株JEM4有一个单一的ospA基因，它与欧洲加氏疏螺旋体菌株PBr（序列同源性值：94.1%）和T25（91.2%）相似。其他七个菌株中的五个在ospA和ospB基因上都表现出同源的C末端（300 bp）（88.7 - 97.3%）。另外两个菌株似乎是由这五个菌株通过ospA或ospB的改变衍生而来。在基于ospA的系统发育分析中，这些菌株可被归类为加氏疏螺旋体，但形成了独立的分支，并与来自欧洲和俄罗斯的典型加氏疏螺旋体分离株分开。
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