Wang J, Masuzawa T, Komikado T, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Microbiol Immunol. 1997;41(2):83-91. doi: 10.1111/j.1348-0421.1997.tb01186.x.
Japanese Lyme borrelias classified as ribotype IV is predominant among isolates derived from clinical specimens, reservoir rodents and Ixodes persulcatus ticks, and has been characterized as Borrelia garinii. These B. garinii isolates have antigenic and genetic features apparently different from North American, European and other Asian isolates, especially in major outer surface proteins A (OspA) and B (OspB). In this study, we cloned and sequenced the genes encoding OspA and OspB from B. garinii strain FujiP2 (ribotype IV strain) isolated from I. persulcatus in Shizuoka, Japan. A sequence analysis revealed significant differences to the previously published sequences of ospA and ospB of B. burgdorferi sensu lato. The open reading frames of ospA and ospB consist of 822 and 888 nucleotides corresponding to the proteins of 273 and 295 amino acids, with molecular weights of 29,643 and 31,786 daltons, respectively. The most interesting finding is that the two osp genes share a consensus 282 bp sequence in their carboxy-terminal portions and that the ospB gene is flanked by a 282 bp-long direct repeat sequence. The deduced amino-acid (aa) sequences of OspA and OspB of strain FujiP2 showed 60.1% homology, and have overall similarities of 70.5%, 70.3% and 75.6% to OspAB proteins of B. burgdorferi sensu stricto strain B31, Borrelia afzelii strain ACA1 and Borrelia garinii strain Ip90, respectively.
在从临床标本、储存宿主啮齿动物和全沟硬蜱分离出的菌株中，分类为核糖体分型IV的日本莱姆疏螺旋体占主导地位，并已被鉴定为伽氏疏螺旋体。这些伽氏疏螺旋体菌株具有明显不同于北美、欧洲和其他亚洲菌株的抗原和遗传特征，尤其是在主要外表面蛋白A（OspA）和B（OspB）方面。在本研究中，我们从日本静冈县全沟硬蜱分离出的伽氏疏螺旋体FujiP2菌株（核糖体分型IV菌株）中克隆并测序了编码OspA和OspB的基因。序列分析显示，与先前发表的狭义伯氏疏螺旋体ospA和ospB序列存在显著差异。ospA和ospB的开放阅读框分别由822和888个核苷酸组成，对应于273和295个氨基酸的蛋白质，分子量分别为29,643和31,786道尔顿。最有趣的发现是，这两个osp基因在其羧基末端部分共享一个282 bp的共有序列，并且ospB基因两侧是一个282 bp长的直接重复序列。FujiP2菌株OspA和OspB的推导氨基酸（aa）序列显示出60.1%的同源性，与狭义伯氏疏螺旋体菌株B31、阿氏疏螺旋体菌株ACA1和伽氏疏螺旋体菌株Ip90的OspAB蛋白的总体相似性分别为70.5%、70.3%和75.6%。
