Naigamwalla D Z, Chaconas G
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1.
EMBO J. 1997 Sep 1;16(17):5227-34. doi: 10.1093/emboj/16.17.5227.
Mu DNA transposition occurs within the context of higher order nucleoprotein structures or transpososomes. We describe a new set of transpososomes in which Mu B-bound target DNA interacts non-covalently with previously characterized intermediates prior to the actual strand transfer. This interaction can occur at several points along the reaction pathway: with the LER, the Type 0 or the Type 1 complexes. The formation of these target capture complexes, which rapidly undergo the strand transfer chemistry, is the rate-limiting step in the overall reaction. These complexes provide alternate pathways to strand transfer, thereby maximizing transposition potential. This versatility is in contrast to other characterized transposons, which normally capture target DNA only at a single point in their respective reaction pathways.
Mu DNA转座发生在高阶核蛋白结构或转座体的背景下。我们描述了一组新的转座体，其中与Mu B结合的靶DNA在实际链转移之前与先前表征的中间体非共价相互作用。这种相互作用可以在反应途径的几个点发生：与LER、0型或1型复合物。这些靶标捕获复合物的形成会迅速经历链转移化学过程，是整个反应中的限速步骤。这些复合物为链转移提供了替代途径，从而使转座潜力最大化。这种多功能性与其他已表征的转座子形成对比，其他转座子通常仅在其各自反应途径中的单个点捕获靶DNA。
