Le Maire V, Hernvann A, Le Marechal H, Ekindjian O G, Aussel C
Laboratoire de Biochimie Cellulaire de l'Inflammation, Université Paris Sud, France.
Cell Biol Int. 1997 Jul;21(7):455-60. doi: 10.1006/cbir.1997.0169.
Given the importance of interleukin-1 in both rheumatic diseases and the modulation of cell metabolic activities, we studied the action of this cytokine on the neutral amino acid transport A system on rheumatoid synovial cells. In these cells IL-1 (1 ng/ml) induced amino transport stimulation from 5 min to 5 h. This effect was obtained only after a starvation period. No concentration-related effect was found for IL-1-stimulated MeAIB uptake, and the IL-1-mediated MeAIB uptake stimulation is independent of protein synthesis. Neosynthesis or post-translational maturation of protein transport is a prerequisite for obtaining this effect. In conclusion, rheumatoid synovial cells exhibit a higher sensitivity for IL-1 than osteoarthritic ones, probably related to their intense metabolic activity.
鉴于白细胞介素-1在风湿性疾病以及细胞代谢活动调节中的重要性，我们研究了这种细胞因子对类风湿性滑膜细胞中性氨基酸转运A系统的作用。在这些细胞中，白细胞介素-1（1纳克/毫升）在5分钟至5小时内诱导氨基酸转运刺激。这种效应仅在饥饿期后出现。白细胞介素-1刺激的α-甲基氨基异丁酸（MeAIB）摄取未发现浓度相关效应，且白细胞介素-1介导的MeAIB摄取刺激与蛋白质合成无关。蛋白质转运的新合成或翻译后成熟是获得这种效应的先决条件。总之，类风湿性滑膜细胞对白细胞介素-1的敏感性高于骨关节炎滑膜细胞，这可能与其强烈的代谢活动有关。
