LeMaire V, Aussel C, Hernvann A, Launay J M, Ekindjian O G
Laboratoire de Biochimie Cellulaire de l'Inflammation, Université Paris Sud Châtenay-Malabry, France.
Eur Cytokine Netw. 1994 Sep-Oct;5(5):449-53.
After one hour incubation with interleukin-1 beta (IL-1 beta), the uptake of alpha-(methylamino) isobutyric acid (MeAIB) by human osteoarthritic synovial cells appeared significantly increased. This effect, observed with 0.1 to 5 ng/ml of cytokine, was inhibited by cycloheximide, indicating that protein synthesis is involved. In addition, this effect seems mediated by a pertussis toxin-sensitive G protein. Finally, intracellular cAMP concentration measurements, the use of a phorbol ester, protein kinase inhibitors and forskolin+3-isobutyl-1-methylxantine (IBMX) provided evidence that a cAMP-dependent protein kinase is associated with interleukin-1 beta-mediated alpha-(methylamino) isobutyric acid uptake.
用人白细胞介素-1β（IL-1β）孵育一小时后，人骨关节炎滑膜细胞对α-（甲基氨基）异丁酸（MeAIB）的摄取明显增加。在0.1至5 ng/ml的细胞因子作用下观察到的这种效应被放线菌酮抑制，表明其涉及蛋白质合成。此外，这种效应似乎由百日咳毒素敏感的G蛋白介导。最后，细胞内cAMP浓度测量、佛波酯的使用、蛋白激酶抑制剂以及福斯高林+3-异丁基-1-甲基黄嘌呤（IBMX）提供了证据，表明cAMP依赖性蛋白激酶与白细胞介素-1β介导的α-（甲基氨基）异丁酸摄取有关。
