甘氨酸促进γ-谷氨酰半胱氨酰乙酯介导的肝脏谷胱甘肽水平升高。

Glycine facilitates gamma-glutamylcysteinylethyl ester-mediated increase in liver glutathione level.

作者信息

Nishida K, Ohta Y, Ishiguro I

机构信息

Department of Biochemistry, School of Medicine, Fujita Health University, Aichi, Japan.

出版信息

Eur J Pharmacol. 1997 Aug 27;333(2-3):289-92. doi: 10.1016/s0014-2999(97)01130-8.

DOI:10.1016/s0014-2999(97)01130-8

PMID:9314046

Abstract

gamma-Glutamylcysteinylethyl ester (gamma-GCE) increases reduced glutathione (GSH) levels in GSH-depleted rat hepatocytes. Because glycine, a constituent of GSH, exists at 0.3 to 0.4 mM in rat plasma, we examined the influence of glycine added to the medium on the action of gamma-GCE to increase GSH levels in the rat hepatocytes. Glycine (0.2-0.8 mM) dose-dependently enhanced gamma-GCE-mediated increase in intracellular GSH levels with an increase in intracellular gamma-GCE levels. These results indicate that exogenous glycine facilitates gamma-GCE-mediated increase in intracellular GSH levels in rat hepatocytes possibly by enhancing the uptake of gamma-GCE into the cells.

摘要

γ-谷氨酰半胱氨酰乙酯（γ-GCE）可提高谷胱甘肽（GSH）耗竭的大鼠肝细胞内还原型谷胱甘肽（GSH）的水平。由于GSH的组成成分甘氨酸在大鼠血浆中的浓度为0.3至0.4 mM，我们研究了向培养基中添加甘氨酸对γ-GCE提高大鼠肝细胞内GSH水平作用的影响。甘氨酸（0.2 - 0.8 mM）呈剂量依赖性地增强了γ-GCE介导的细胞内GSH水平的升高，同时细胞内γ-GCE水平也有所增加。这些结果表明，外源性甘氨酸可能通过增强γ-GCE进入细胞的摄取，促进了γ-GCE介导的大鼠肝细胞内GSH水平的升高。