Allen E D, Weatherbee L
J Microsc. 1979 Dec;117(3):381-94. doi: 10.1111/j.1365-2818.1979.tb04695.x.
The freeze fracture appearance of red cells frozen in the presence of varying concentrations of hydroxyethyl starch (HES) is described. A technique is used which allows examination of a small portion of cells from a larger unit. The frozen cells appear distorted probably as a result of osmotic dehydration but indicate no evidence of intracellular ice. The frozen mixture with HES has three phases--a particulate phase consisting of the concentrated HES (and other salts), a sculptured ice phase and the red cells. When the concentration of HES is increased, the particulate phase becomes more prominent and at 14% HES appears to surround nearly all cells. In cells frozen in saline alone and 4% HES, the cytoplasm in a majority of cells has numerous cavities and depressions. Since such units haemolyse badly when thawed, it is possible that these regions indicate structural damage. In contrast, those units frozen with 14% HES (in which nearly 85% of the cells survive freeze-thaw) possess cells which only infrequently have such regions in the cytoplasm.
本文描述了在不同浓度羟乙基淀粉（HES）存在下冷冻的红细胞的冷冻断裂外观。采用了一种技术，该技术允许检查来自较大单元的一小部分细胞。冷冻的细胞似乎因渗透脱水而变形，但没有细胞内结冰的迹象。含有HES的冷冻混合物有三个相——由浓缩的HES（和其他盐类）组成的颗粒相、雕刻的冰相和红细胞。当HES的浓度增加时，颗粒相变得更加突出，在14%的HES浓度下似乎几乎包围了所有细胞。在仅在盐水中冷冻和在4% HES中冷冻的细胞中，大多数细胞的细胞质有许多空洞和凹陷。由于这些单元在解冻时严重溶血，这些区域可能表明存在结构损伤。相比之下，用14% HES冷冻的那些单元（其中近85%的细胞在冻融后存活）所拥有的细胞，其细胞质中很少有这样的区域。
