Sternberg C, Eberl L, Poulsen L K, Molin S
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
J Biolumin Chemilumin. 1997 Jan-Feb;12(1):7-13. doi: 10.1002/(SICI)1099-1271(199701/02)12:1<7::AID-BIO427>3.0.CO;2-3.
Detection of very low light levels arising from individual cells of the naturally bioluminescent bacterium Vibrio fischeri as well as from a luminescence-marked Pseudomonas putida strain was achieved by the aid of two different camera systems. Using a liquid nitrogen-cooled slow-scan CCD (charge-coupled device) camera were able to detect single-cell bioluminescence within 1 min, and the pictures obtained were of good resolution. In contrast, employing a photon-counting video camera we were able to detect bioluminescent cells within 10 seconds, but at the expense of spatial resolution. This study demonstrates the feasibility of microscopic single cell analysis employing bioluminescence as reporter system.
借助两种不同的摄像系统，实现了对天然生物发光细菌费氏弧菌单个细胞以及发光标记的恶臭假单胞菌菌株产生的极低光水平的检测。使用液氮冷却的慢扫描电荷耦合器件（CCD）相机，我们能够在1分钟内检测到单细胞生物发光，并且获得的图像分辨率良好。相比之下，使用光子计数摄像机，我们能够在10秒内检测到生物发光细胞，但牺牲了空间分辨率。本研究证明了采用生物发光作为报告系统进行微观单细胞分析的可行性。
