Akulinin V A, Stepanov S S, Semchenko V V, Belichenko P V
Department of Histology and Embryology, Medical Academy, Omsk, Russia.
Resuscitation. 1997 Oct;35(2):157-64. doi: 10.1016/s0300-9572(97)00048-8.
Experiments were performed on 40 Wistar rats. Total brain ischemia was induced by 10 min clamping of the cardiac blood vessels. The brains were examined in control rats, after 90 min and after 1, 3, 7, 30 and 90 days during the postresuscitation period. Histological sections were stained with the Golgi method. Morphometrical parameters, 12, of dendritic changes of the pyramidal neurons in layer V of sensory motor cortex (SMC) in rat brain were studied at different intervals of the postresuscitation period. Reduction of the dendrites of the pyramidal neurons due to the loss of the terminal branches of the oblique apical dendrites in layer III-IV was detected from the first day after ischemia. The maximal dendritic change was detected 3 and 7 days after ischemia. Decrease the volume of dendritic territory (54, 4%), the total dendritic length of the whole dendritic territory (56, 0%) and branching of dendrites, and decrease in the number of dendritic spines on apical dendrites in layers I-II (46, 1%) were the main changes during this period. Reduction of the total length of dendrites occurred mostly due to disappearance of the 2nd and 3rd order branches of the apical and basal dendrites. The change of dendrites neurons had returned to control levels after 30 days. By that time the diameter of the dendrites had increased, the varicosities on oblique apical and basal dendrites had disappeared, and the number of 2nd and 3rd order dendrites and of dendritic spines had increased.
对40只Wistar大鼠进行了实验。通过钳夹心脏血管10分钟诱导全脑缺血。在复苏后期间，对对照组大鼠以及缺血90分钟后、1天、3天、7天、30天和90天后的大鼠大脑进行检查。组织学切片采用高尔基法染色。研究了大鼠脑感觉运动皮层（SMC）第V层锥体细胞树突变化的12个形态学参数在复苏后不同时间段的情况。缺血后第一天就检测到锥体细胞树突因III - IV层斜顶树突终末分支的丧失而减少。缺血后3天和7天检测到最大的树突变化。在此期间的主要变化包括树突区域体积减少（54.4%）、整个树突区域的总树突长度减少（56.0%）、树突分支减少以及I - II层顶树突上树突棘数量减少（46.1%）。树突总长度的减少主要是由于顶树突和基底树突的二级和三级分支消失。30天后树突神经元的变化已恢复到对照水平。到那时，树突直径增加，斜顶树突和基底树突上的膨体消失，二级和三级树突以及树突棘的数量增加。
