Takasaki J, Ogawa Y
Department of Pediatrics, Saitama Medical Center, Saitama Medical School, Japan.
Acta Paediatr Jpn. 1997 Aug;39(4):409-12. doi: 10.1111/j.1442-200x.1997.tb03607.x.
Measurements were made of the granulocyte elastase activity and the concentration of granulocyte elastase alpha 1-proteinase inhibitor complex (E-alpha 1 PI) in the cerebrospinal fluid (CSF) of patients with purulent meningitis and aseptic meningitis. Granulocyte elastase activity was determined by the cleavage of methoxysuccinyl-L-(alanyl)2-prolyl-valyl-p-nitroanilide peptide substrate. The E-alpha 1 PI concentration was assayed with an enzyme immunoassay technique. Granulocyte elastase activity in the CSF on admission was significantly higher in patients with purulent meningitis (800 +/- 539 micrograms/L; mean +/- SD) than in patients with aseptic meningitis (< 10 micrograms/L). Granulocyte elastase activity in the CSF of patients with purulent meningitis decreased rapidly except for two patients who subsequently developed hydrocephalus. There was a weak correlation between granulocyte elastase activity and the concentration of E-alpha 1 PI in the CSF with purulent meningitis (n = 67, r = 0.48, P < 0.01). However, some samples of CSF showed a marked discrepancy between granulocyte elastase activity and E-alpha 1 PI concentration, indicating the importance of measuring granulocyte elastase activity in the CSF to estimate tissue injury.
对化脓性脑膜炎和无菌性脑膜炎患者的脑脊液（CSF）中的粒细胞弹性蛋白酶活性以及粒细胞弹性蛋白酶α1-蛋白酶抑制剂复合物（E-α1PI）浓度进行了测量。粒细胞弹性蛋白酶活性通过甲氧基琥珀酰-L-（丙氨酰）2-脯氨酰-缬氨酰-对硝基苯胺肽底物的裂解来测定。E-α1PI浓度采用酶免疫测定技术进行测定。化脓性脑膜炎患者入院时脑脊液中的粒细胞弹性蛋白酶活性（800±539微克/升；均值±标准差）显著高于无菌性脑膜炎患者（<10微克/升）。除两名随后发生脑积水的患者外，化脓性脑膜炎患者脑脊液中的粒细胞弹性蛋白酶活性迅速下降。化脓性脑膜炎患者脑脊液中粒细胞弹性蛋白酶活性与E-α1PI浓度之间存在弱相关性（n = 67，r = 0.48，P < 0.01）。然而，一些脑脊液样本显示粒细胞弹性蛋白酶活性与E-α1PI浓度之间存在明显差异，这表明测量脑脊液中的粒细胞弹性蛋白酶活性对于评估组织损伤的重要性。
