Green K, Downs S, Bowman K
Invest Ophthalmol. 1976 Jun;15(6):484-6.
The glycosaminoglycans of isolated rabbit corneal stroma, clamped between two lucite plates at near normal hydration, were digested with testicular hyaluronidase in saline solution. After equilibration with 0.9 per cent saline solution alone the sodium and chloride content of the stroma was determined. Chloride was in equilibrium with both normal and hyaluronidase-treated stroma, allowing use of the Donnan calculation for excess or bound sodium to be made. Normal stromas contained 200 mEq. bound sodium per kilogram of dry weight calculated from the Donnan calculation; hyaluronidase-treated stromas contained 110 mEq. bound sodium per kilogram of dry weight. The data show that about half of the bound sodium in the corneal stroma is on nonsaccharide binding sites. Quantitative verification of the loss of glycosaminoglycans was performed.
将分离出的兔角膜基质夹在两块有机玻璃片之间，在接近正常水合状态下，于盐溶液中用睾丸透明质酸酶进行消化。仅用0.9%盐溶液平衡后，测定基质中的钠和氯含量。氯在正常基质和经透明质酸酶处理的基质中均处于平衡状态，这使得可以利用唐南计算法来计算过量或结合的钠。根据唐南计算法，正常基质每千克干重含有200毫当量的结合钠；经透明质酸酶处理的基质每千克干重含有110毫当量的结合钠。数据表明，角膜基质中约一半的结合钠位于非糖结合位点上。对糖胺聚糖的损失进行了定量验证。
