Millero F J, Ward G K, Chetirkin P
J Biol Chem. 1976 Jul 10;251(13):4001-4.
Density measurements have been made on aqueous lysozyme solutions at 20, 25, and 30 degrees. The apparent specific volumes, phi v, and expansibilities, phi e, have been determined from the density measurements and fitted to a function of concentration (weight per cent). Sound velocities and heat capacities have also been measured for various concentrations of lysozyme-water solutions at 25 degrees. From the density, expansibility, heat capacity, and sound velocity data at 25 degrees, the isothermal compressibility, phi k, for the lysozyme solutions have been calculated over a range of concentrations. All the physicochemical properties measured were found to be a linear function of the weight per cent of lysozyme. The number of water molecules hydrated to 1 mol of lysozyme was estimated from the volume and compressibility and found to be 162 at 25 degrees.
已在20、25和30摄氏度下对溶菌酶水溶液进行了密度测量。根据密度测量确定了表观比容（ϕv）和膨胀系数（ϕe），并将其拟合为浓度（重量百分比）的函数。还在25摄氏度下测量了不同浓度的溶菌酶 - 水溶液的声速和热容量。根据25摄氏度下的密度、膨胀系数、热容量和声速数据，计算了一系列浓度范围内溶菌酶溶液的等温压缩率（ϕk）。发现所有测量的物理化学性质都是溶菌酶重量百分比的线性函数。根据体积和压缩率估计了与1摩尔溶菌酶结合的水分子数量，发现在25摄氏度下为162个。
