大鼠精子发生过程中类核体对（3H）尿苷的摄取
Incorporation of (3H)uridine by the chromatoid body during rat spermatogenesis.
作者信息
Söderström K O, Parvinen M
出版信息
J Cell Biol. 1976 Jul;70(1):239-46. doi: 10.1083/jcb.70.1.239.
Abstract
The in vitro incorporation of tritiated uridine into RNA by the spermatogenic cells of the rat has been analyzed by high-resolution autoradiography. Special attention has been focused on the unique cytoplasmic organelle, the chromatoid body. After a short labeling time (2 h), this organelle remains unlabeled in the vast majority of the early spermatids although the nuclei are labeled. When the 2-h incubation with (3H)uridine is followed by a 14-h chase, the chromatoid body is seen distinctly labeled in all spermatids during early spermiogenesis from step 1 to step 8. Very few grains are seen elsewhere in the cytoplasm of these cells. When RNA synthesis in the spermatid ceases, the chromatoid body also remains unlabeled. It is likely that the chromatoid body contains RNA which is synthesized in the nuclei of the spermatids. The function of this RNA as a stable messenger RNA needed for the regulation of late spermiogenesis is discussed.
摘要
通过高分辨率放射自显影分析了大鼠生精细胞将氚标记的尿苷体外掺入RNA的情况。特别关注了独特的细胞质细胞器——类核体。在短时间标记（2小时）后，尽管细胞核被标记，但在绝大多数早期精子细胞中，这种细胞器仍未被标记。当用（3H）尿苷孵育2小时后再进行14小时的追踪时，在精子发生早期从第1步到第8步的所有精子细胞中，类核体都明显被标记。在这些细胞的细胞质其他部位几乎看不到银粒。当精子细胞中的RNA合成停止时，类核体也仍未被标记。类核体可能含有在精子细胞核中合成的RNA。讨论了这种RNA作为调节精子发生后期所需的稳定信使RNA的功能。
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