Sakurai H, Ishihama A
National Institute of Genetics, Department of Molecular Genetics, Shizuoka, Japan.
Gene. 1997 Sep 1;196(1-2):165-74. doi: 10.1016/s0378-1119(97)00222-9.
RNA polymerase II purified from the fission yeast Schizosaccharomyces pombe contains 10 different species of polypeptides. Previously, we cloned and sequenced both cDNA and the genes encoding the four large subunits, Rpb1, Rpb2, Rpb3 and Rpb5. Later, other groups isolated the genes for Rpb6 and Rpb12 and cDNA for Rpb10. Here, we cloned both cDNA and the genes encoding four small subunits, Rpb7, Rpb8, Rpb10 and Rpb11. These genes were found to encode Rpb7, Rpb8, Rpb10 and Rpb11 consisting of 172 (19,103 Da), 125 (14,300 Da), 71 (8276 Da) and 123 (14,127 Da) amino acid residues, respectively. All these four subunits are homologous to the corresponding subunits of Saccharomyces cerevisiae RNA polymerase II. The rpb7 gene contains one intron, whereas the rpb8, rpb10 and rpb11 genes contain two introns. Taken altogether, the gene organization and the predicted protein sequence have been determined for all 10 subunits of the S. pombe RNA polymerase II.
从裂殖酵母粟酒裂殖酵母中纯化得到的RNA聚合酶II含有10种不同的多肽。此前，我们克隆并测序了编码四个大亚基Rpb1、Rpb2、Rpb3和Rpb5的cDNA和基因。后来，其他研究小组分离出了Rpb6和Rpb12的基因以及Rpb10的cDNA。在此，我们克隆了编码四个小亚基Rpb7、Rpb8、Rpb10和Rpb11的cDNA和基因。这些基因分别编码由172个（19,103道尔顿）、125个（14,300道尔顿）、71个（8276道尔顿）和123个（14,127道尔顿）氨基酸残基组成的Rpb7、Rpb8、Rpb10和Rpb11。所有这四个亚基都与酿酒酵母RNA聚合酶II的相应亚基同源。rpb7基因含有一个内含子，而rpb8、rpb10和rpb11基因含有两个内含子。总之，已经确定了粟酒裂殖酵母RNA聚合酶II所有10个亚基的基因结构和预测的蛋白质序列。
