Zou J, Taylor D C
National Research Council of Canada, Plant Biotechnology Institute, Saskatchewan, Canada.
Gene. 1997 Sep 1;196(1-2):291-5. doi: 10.1016/s0378-1119(97)00258-8.
The RING (Really Interesting New Gene) finger is a zinc-binding domain that is found in proteins from a variety of species. This paper reports the cloning and characterization of, as yet, only the second RING finger protein gene from plants, A-RZE, in Arabidopsis thaliana. In addition to the RING-finger motif, A-RZF also contains a putative nuclear localization signal. A-RZF is encoded by a single copy gene with an intron of 595 bp interrupting the 5' leader sequence and the coding region. Northern blot analysis indicated that A-RZF is expressed preferentially during seed development. The RING-finger motif, putative nuclear localization signal, and unique expression pattern, predict an important role during seed development for A-RZF.
RING（真有趣新基因）结构域是一种锌结合结构域，存在于多种物种的蛋白质中。本文报道了拟南芥中迄今发现的第二个植物RING结构域蛋白基因A-RZE的克隆和特性分析。除了RING结构域基序外，A-RZF还包含一个假定的核定位信号。A-RZF由一个单拷贝基因编码，该基因有一个595 bp的内含子，中断了5'前导序列和编码区。Northern杂交分析表明，A-RZF在种子发育过程中优先表达。RING结构域基序、假定的核定位信号和独特的表达模式，预示着A-RZF在种子发育过程中起重要作用。
