Nishii A, Takemura M, Fujita H, Shikata M, Yokota A, Kohchi T
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
Biosci Biotechnol Biochem. 2000 Jul;64(7):1402-9. doi: 10.1271/bbb.64.1402.
By differential screening of an arrayed normalized cDNA library from the inflorescence apex in Arabidopsis, a cDNA clone having a deduced amino acid sequence with a motif for a zinc finger was isolated as one of the genes expressed specifically in the reproductive phase. The deduced protein has a modular structure with a putative single C2-C2 zinc-finger motif distantly related to a GATA-1-type finger, a basic region with a sequence resembling a nuclear localization signal, and an acidic region. The gene seemed to have been formed by the exon-shuffling during its molecular evolution, since individual domains are encoded by discrete exons. RNA gel blot analysis showed its expression in shoot apex and flowers in the reproductive phase. The gene was named ZIM for Zinc-finger protein expressed in Inflorescence Meristem. The nuclear localization of ZIM was detected using GFP as a reporter. These results suggest that ZIM is a putative transcription factor involved in inflorescence and flower development.
通过对拟南芥花序顶端的阵列归一化cDNA文库进行差异筛选，分离出一个cDNA克隆，其推导的氨基酸序列带有锌指基序，作为在生殖阶段特异性表达的基因之一。推导的蛋白质具有模块化结构，带有一个与GATA - 1型指远缘相关的假定单C2 - C2锌指基序、一个具有类似核定位信号序列的碱性区域和一个酸性区域。由于各个结构域由离散的外显子编码，该基因似乎是在其分子进化过程中通过外显子重排形成的。RNA凝胶印迹分析表明其在生殖阶段的茎尖和花中表达。该基因被命名为ZIM，即花序分生组织中表达的锌指蛋白。使用绿色荧光蛋白作为报告基因检测到ZIM的核定位。这些结果表明ZIM是一种参与花序和花发育的假定转录因子。
