Li R, Mather J P
Cell Biology, Genentech, Inc., South San Francisco, CA 94080, USA.
Endocrinology. 1997 Oct;138(10):4477-80. doi: 10.1210/endo.138.10.5567.
Folliculogenesis in the ovary begins before birth with the formation of primordial follicles consisting of an oocyte in the resting meiotic prophase surrounded by a layer of undifferentiated granulosa cells. At each menstrual cycle a subset of these follicles is selected and undergoes growth and differentiation leading to ovulation and corpora lutea formation or apoptosis. We have previously described an in vitro model of rat follicular development in which the organization of three-dimensional antral-follicles requires FSH and activin A. Here we report that, in this system, the oocyte is required for follicle morphogenesis and requires gap junctions to direct the morphological arrangement of the surrounding granulosa cells.
卵巢中的卵泡发生在出生前就开始了，原始卵泡形成，其由处于减数分裂前期静止期的卵母细胞和一层未分化的颗粒细胞组成。在每个月经周期中，这些卵泡的一个子集被选中并经历生长和分化，导致排卵和黄体形成或凋亡。我们之前描述了一种大鼠卵泡发育的体外模型，其中三维窦状卵泡的组织需要促卵泡激素（FSH）和激活素A。在此我们报告，在这个系统中，卵泡形态发生需要卵母细胞，并且需要缝隙连接来指导周围颗粒细胞的形态排列。
