Gargano N, Cattaneo A
MRC Laboratory of Molecular Biology, Cambridge, UK.
FEBS Lett. 1997 Sep 15;414(3):537-40. doi: 10.1016/s0014-5793(97)01065-x.
The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this reason, it would be desirable to exploit intracellular expression of antibodies to select or rescue more efficient ones from a polyclonal mixture. In this work we have successfully explored this possibility by rescuing a neutralizing anti-viral antibody fragment from an intracellularly expressed anti-reverse transcriptase polyclonal repertoire.
在哺乳动物细胞中重组抗体的细胞内表达是一种在体内抑制所选分子功能的实验策略。然而，该技术的一个局限性表现为抗体在细胞内表达条件下的不可预测行为。因此，利用抗体的细胞内表达从多克隆混合物中选择或挽救更有效的抗体将是很有必要的。在这项工作中，我们通过从细胞内表达的抗逆转录酶多克隆库中挽救一种中和抗病毒抗体片段，成功探索了这种可能性。
