Cardinale A, Lener M, Messina S, Cattaneo A, Biocca S
Department of Neuroscience, University of Rome Tor Vergata, Roma, Italy.
FEBS Lett. 1998 Nov 20;439(3):197-202. doi: 10.1016/s0014-5793(98)01369-6.
The anti-p21ras Y13-259 single-chain Fv fragment (scFv) neutralizes the activity of p21-ras when intracellularly expressed in different systems. We have studied the mode of action of this inhibition in 3T3 K-ras fibroblasts and demonstrated that (i) this antibody fragment is highly aggregating when cytoplasmically expressed and (ii) the p21-ras antigen is sequestered in these aggregates in an antibody-dependent manner. This co-segregation leads to an efficient inhibition of DNA synthesis. These results suggest that an antigen can be diverted from its normal location inside the cells in an antibody mediated way, prospecting a new mode of action for intracellular antibodies in vivo.
抗p21ras Y13 - 259单链Fv片段（scFv）在不同系统中细胞内表达时可中和p21 - ras的活性。我们研究了这种抑制作用在3T3 K - ras成纤维细胞中的作用方式，并证明：（i）该抗体片段在细胞质中表达时高度聚集；（ii）p21 - ras抗原以抗体依赖的方式被隔离在这些聚集体中。这种共分离导致DNA合成的有效抑制。这些结果表明，抗原可以通过抗体介导而从细胞内的正常位置被转移，为体内细胞内抗体的作用方式开辟了新途径。
