Alderson E, Birchall J P, Owen J J
J Immunol Methods. 1976;11(3-4):297-301. doi: 10.1016/0022-1759(76)90123-x.
We present a simple technique for obtaining cell suspension from human peripheral venous blood containing on average 96% lymphocytes, no macrophages and leukocyte red blood cell ratio 1 : 1. Cells are readily prepared under sterile conditions and can be used in culture experiments. The high yield of lymphocytes and the avoidance of Ficoll-Triosil makes the technique especially valuable for micro cytotoxicity tests such as those aimed at measuring tumour immunity.
