Goldrosen M H, Formeister J, Holyoke E D
J Immunol Methods. 1976;11(3-4):367-9. doi: 10.1016/0022-1759(76)90130-7.
This communication provides information on the design and use of a counting machine that enumerates adherent cells in cytotoxicity assays. To date, investigators have relied on inverted microscopes or quantimat automatic image analysis. The former method is tedious and time consuming while the latter method is prohibitively expensive. The counting machine which consists of projection microscope attached to an electronic counter is as accurate as the inverted microscope but is both less tedious and less time consuming.
本通讯提供了一种用于细胞毒性试验中对贴壁细胞进行计数的计数机的设计及使用信息。迄今为止，研究人员一直依赖倒置显微镜或定量自动图像分析。前一种方法繁琐且耗时，而后一种方法成本过高。由连接电子计数器的投影显微镜组成的计数机与倒置显微镜一样准确，但操作起来既不那么繁琐，耗时也更少。
