Hoffmann Johannes J M L, Janssen Willy C M
Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands.
Clin Chem Lab Med. 2002 Nov;40(11):1168-73. doi: 10.1515/CCLM.2002.204.
Counting of cells in cerebrospinal fluid is currently performed manually. Because of the inherent analytical and economical disadvantages, we attempted to introduce a fully automated method. Therefore, we validated the Abbott CellDyn-4000 haematology analyser for counting cells in cerebrospinal fluid. The analyser was used in its standard configuration with the simple precaution of a preceding blank sample. As for leukocyte counting the analyser yielded high precision (CV approximately 5% above the upper reference limit), good linearity, low limit of detection (2/microl) and excellent correlation (r > 0.99) with the counting chamber method. The differential leukocyte count was equally accurate and precise, even in the low concentration range. Performance of the erythrocyte count was impaired by its high limit of detection (6/nl) and it appeared satisfactory only for detecting blood admixture due to traumatic puncture. The specificity of the analyser is excellent, since it correctly classified non-viable leukocytes and excluded yeast cells from the leukocyte count in a patient with cryptococcal meningitis. We conclude that the CellDyn-4000 is well suited for quickly and reliably counting leukocytes in cerebrospinal fluid. Developing some software modifications might make the analyser useful also for performing erythrocyte counting in cerebrospinal fluid.
目前，脑脊液细胞计数是通过手工进行的。由于存在固有的分析和经济方面的劣势，我们尝试引入一种全自动方法。因此，我们对雅培CellDyn - 4000血液分析仪进行了脑脊液细胞计数的验证。该分析仪采用标准配置使用，只需对空白样本进行简单预处理。对于白细胞计数，该分析仪具有高精度（在上限参考值以上CV约为5%）、良好的线性、低检测限（2/微升）以及与计数板法的出色相关性（r>0.99）。即使在低浓度范围内，白细胞分类计数同样准确和精确。红细胞计数的性能因其高检测限（6/纳升）而受到影响，仅在检测因穿刺损伤导致的血液混入时表现令人满意。该分析仪的特异性极佳，因为它能正确区分无活力的白细胞，并在一名患有隐球菌性脑膜炎的患者的白细胞计数中排除酵母细胞。我们得出结论，CellDyn - 4000非常适合快速且可靠地进行脑脊液白细胞计数。开发一些软件修改可能会使该分析仪也适用于脑脊液红细胞计数。
